U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing
نویسندگان
چکیده
منابع مشابه
Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing.
Nuclear pre-messenger RNA (pre-mRNA) splicing requires multiple spliceosomal small nuclear RNA (snRNA) and pre-mRNA rearrangements. Here we reveal a new snRNA conformational switch in which successive roles for two competing U2 helices, stem IIa and stem IIc, promote distinct splicing steps. When stem IIa is stabilized by loss of stem IIc, rapid ATP-independent and Cus2p-insensitive prespliceos...
متن کاملThe structure of an essential splicing element: stem loop IIa from yeast U2 snRNA.
BACKGROUND Eukaryotic genes are usually transcribed as precursor mRNAs which are then spliced, removing introns to produce functional mRNAs. Splicing is performed by the spliceosome and provides an important level of post-translational control of gene expression. Stem loop IIa from U2 small nuclear (sn)RNA is required for the efficient association of the U2 small nuclear ribonuclear protein (sn...
متن کاملtrans-splicing to spliceosomal U2 snRNA suggests disruption of branch site-U2 pairing during pre-mRNA splicing.
Pairing between U2 snRNA and the branch site of spliceosomal introns is essential for spliceosome assembly and is thought to be required for the first catalytic step of splicing. We have identified an RNA comprising the 5' end of U2 snRNA and the 3' exon of the ACT1-CUP1 reporter gene, resulting from a trans-splicing reaction in which a 5' splice site-like sequence in the universally conserved ...
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Activation of pre-messenger RNA (pre-mRNA) splicing requires 5' splice site recognition by U1 small nuclear RNA (snRNA), which is replaced by U5 and U6 snRNA. Here we use crosslinking to investigate snRNA interactions with the 5' exon adjacent to the 5' splice site, prior to the first step of splicing. U1 snRNA was found to interact with four different 5' exon positions using one specific seque...
متن کاملon the equivalence of constructed-response and multiple-choice : stem-equivalent, stem non-equivalent but content equivalent, and stem and content non-equivalent items in reading comprehension using multifaceted rasch
the present study investigated construct equivalence of multiple choice (mc) and constructed response (cr) item types across stem and content equivalent mc and cr items (item type ‘a’), non-stem-equivalent but content equivalent mc and cr items (item type ‘b’), and non-stem and non-content equivalent mc and cr items (item type ‘c’). one hundred seventy english-major undergraduates completed mc ...
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ژورنال
عنوان ژورنال: Genes & Development
سال: 2007
ISSN: 0890-9369
DOI: 10.1101/gad.1536107